| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wachauf, Caleb J (12)
|
NMYWSC |
26.89 |
26.30 |
-0.59 |
| 2 |
Rising, London A (12)
|
MILCY |
27.85 |
26.48 |
-1.37 |
| 3 |
Hicks, Brody (12)
|
LCE |
28.87 |
27.25 |
-1.62 |
| 4 |
McCuiston, Charlie H (12)
|
MIBBD |
28.29 |
27.35 |
-0.94 |
| 5 |
Mihok, Zachary T (12)
|
LEYYN |
28.93 |
28.12 |
-0.81 |
| 6 |
Barasch, Nathan T (12)
|
OHFMST |
29.73 |
29.41 |
-0.32 |
| 7 |
Boh, Andrew M (12)
|
OHRCDB |
29.42 |
29.42 |
0.00 |
| 8 |
Hansen, Braylon (12)
|
INFWCY |
30.19 |
29.93 |
-0.26 |
| 9 |
Kibler, Carter F (12)
|
LEYYN |
30.61 |
30.36 |
-0.25 |
| 10 |
Holter, Robert W (11)
|
MIRAYS |
30.36 |
30.61 |
0.25 |
| 11 |
O'Neill, Quinn K (12)
|
MIBBD |
31.17 |
31.12 |
-0.05 |
| 12 |
Yapchai, Bryce A (11)
|
MIFLY |
31.25 |
31.30 |
0.05 |
| 13 |
Summers, Grady (11)
|
AYAA |
31.79 |
31.74 |
-0.05 |
| 13 |
Novak, Will P (11)
|
LECFYN |
31.76 |
31.74 |
-0.02 |
| 15 |
McGraw, Parker W (12)
|
OHPUT |
32.04 |
32.21 |
0.17 |
| 16 |
Turney, Reed T (11)
|
LECFYN |
34.64 |
32.35 |
-2.29 |
| 17 |
Askren, Caden (12)
|
INYOGI |
31.86 |
32.39 |
0.53 |
| 18 |
Baldauf, Andrew (12)
|
OHLYSC |
32.65 |
32.41 |
-0.24 |
| 19 |
Latham, Mason F (12)
|
OHLYSC |
32.71 |
32.58 |
-0.13 |
| 20 |
DiStefano, Samuel D (12)
|
LEYYN |
32.46 |
32.64 |
0.18 |
| 21 |
Baker, Elijah (11)
|
INFWCY |
32.14 |
32.98 |
0.84 |
| 22 |
Nelson, Carter (11)
|
AYAA |
35.44 |
33.91 |
-1.53 |
| 23 |
Swineheart, Nathan W (11)
|
INFWCY |
34.20 |
34.38 |
0.18 |
| 24 |
Zhang, Allen Z (12)
|
MIBBD |
31.65 |
34.39 |
2.74 |